Description

Size

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For Electrophoresis
In this bottle(375mL), just add 125mL of ddH₂O to make 500mL of 30% solution!

In Tri-Distilled Water, 0.45um Membrane Filtered.
19:1 (Acrylamide:Bis = 38%:2%)
29:1 (Acrylamide:Bis = 38.67%:1.33%)
37.5:1 (Acrylamide:Bis = 38.96%:1.04%)

Store at 4℃

Caution: Acrylamide is a potent neurotoxin and absorbed through the skin. The effects of acrylamide are cumulative. Although polyacrylamide is suspected to be nontoxic, it should be handled with great care because of the possibility that it might contain small quantities of unpolymerized acrylamide.

Toxic, Carcinogenic, Mutagenic, Irritant, Sensitizing, Toxic for reproduction

Selection Antibiotics

Dactinomycin
C₆₂H₈₆N₁₂O₁₆
M.W.: 1255.42
95% Min. (HPLC)
USP 23 stable substance.

Actinomycin D intercalates into DNA and thereby inhibits the transcription. It binds preferentially to guanine and blocks the RNA polymerase during the elongation step. A high dose (2ug/mL, 1.59uM) inhibits the transcription of all RNA species, lower concentrations (40ng/mL, 32nM) inhibit the synthesis of rRNA.

Stock Conc.: 5mg/mL in MeOH/EtOH
Working Conc.: 0.04-50µg/mL

Store at 4℃

Very toxic!

Highly recommended for monitoring total protein load on Western blots.

Comments: Ab-5 reacts with all six known vertebrate isoforms of actin (M.W. ~42kDa). It also reacts with two cytoplasmic actins (Beta, Gamma) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues.

Applications and Suggested Dilutions:
- Immunoprecipitation (Denatured verified) (Use Protein G) (Ab 2ug/mg protein lysate)
- Western Blotting (0.5ug/mL for 2hrs at RT)
- Immunohistology (formalin/paraffin) (Use at 1:400 for 30 min. [Staining of formalin-fixed, paraffin-embedded tisssues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min.]

Store at 4℃

Molecular Biology Buffer

N-(2-Acetamido)-Iminodiacetic Acid
C₆H₁₀N₂O₅
M.W.: 190.15

Hydrogen ion buffers for biological research.
The free acid of ADA is of low solubility. The mono- or disodium salt is readily soluble at pH 6.4 - 7.4. Dissolve the free acid in equimolar sodium hydroxide.

Good, N.E. et al. (1966) Biochemistry 5, 467-477

Store at Room Temp.

Protein Experiment, Protease Inhibitor, Low Toxicity

4-(2-AminoEthyl)-BenzeneSulfonyl Fluoride HCl
C₈H₁₀FNO₂S·HCl
M.W.: 239.69

Less toxic alternative to PMSF, pH stable.
AEBSF irreversibly inactivates thrombin and other serine proteases (e. g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme. AEBSF (LD₅₀ oral, mouse 2.8g/kg) is a substantially less toxic substitute for PMSF and DFP.

Stock Conc.: 20-100mM in water or buffer, -20℃, stable for 2 months
Working Conc.: 0.1-2mM

Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-85.

Store at 4℃

For Microbiology Culture

Loss on drying: <10%
Residue on Ignition: <5%
Gel strength:  900g/cm²
Ash, Total : NMT6.5%
Heavy metals: <40ppm
Lead: <10ppm

For the preparation of 1 Liter liquid for LB agar plates weigh 10 g tryptone, 5 g yeast extract, 5 g NaCl and 15 g agar. Add 1 ml 1 N NaOH to the dissolved powder. The medium will be autoclaved for 25 minutes. At a temperature of ~50℃, it is still liquid and additives (e. g. antibiotics) can be added. If 32 to 40 ml LB agar per plate are used, one liter will result in approx. 25 to 30 plates. Freshly poured plates cool down with a slightly opened lid and turn them upside down when stored at +4℃.

Ausubel, F.A.. Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl. K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience. New York.

Store at Room Temp.

For Electrophoresis, High gel strength
Instruction for Gel Preparation

EEO(-m): 0.1-0.15
Sulfate: 0.15-0.2%
Gel Strength (1% gel): 1200g/cm² Min.
Gelling Point (1.5% gel): 33 ± 1.5℃
Melting Point (1.5% gel): 87 ± 1.5℃

Store at Room Temp.

For Electrophoresis, Low Melting Agarose, Genetic Quality Tested

Electrophoresis of DNA fragments ≥ 1000bp. In-gel enzymatic digestion, ligation, PCR. Preparative Electrophoresis. Analysis and recovery of larger DNA-fragments for further application.

Store at Room Temp.

Protein Experiment, For Protein Purification

H₈N₂O₄S
M.W.: 132.14

Ammonium sulfate is used for the selective precipitation of proteins.

Wood, W.I. (1976) Anal. Biochem. 73, 250-257

Store at Room Temp.

Fungizone, Selection Antibiotics, for Fungi and Yeast.

C₄₇H₇₃NO₁₇
M.W.: 924.08　
USP

Freez dryed, non-sterile
Additive: Sodiumdeoxyclolate(41mg) and phospate buffers

The antibiotic amphotericin B was isolated from Streptomyces nodosus. It belongs to the macrocyclic lactones and its action is fungistatic. Amphotericin B binds to sterols (e. g. cholesterol, cholestanol) with planar structure and disturbs the membrane permeability. Ions, like K+, Na+ and H+ or other low molecular weight substances (e. g. amino acids, sugars, nucleotides) may cross the membrane.

Stock Conc.: 30-40mg/mL in DMSO
Working Conc.: 0.25-2.5ug/mL

Store at 4℃

Harmful

Selection Antibiotics

D(-)-Alpha-Aminobenzylpenicilline Sodium Salt
C₁₆H₁₈N₃O₄SNa
M.W.: 371.39
99% Min.
DAB10

Solubility in water: >20g/100mL
Tested in cell culture and molecular biology.

Stock Conc.: 50 mg/mL in water, Store at -20℃
Working Conc.: 20-60 ug/mL

Store at 4℃

Selection Antibiotics
Nebramycin II

C₂₁H₄₁N₅O₁₁·H₂SO₄
M.W.: 637.66

Aminoglycoside antiobitic with bactericidal activity against most gram negative bacteria.

Store at 4℃

For Bioluminescent Assay
Are you searching for dATP?

Adenosine-5'-Triphosphate Disodium Salt Trihydrate
C₁₀H₁₄N₅Na₂O₁₃P₃
M.W.: 551.14
96% Min.
ADP: <1.5% (HPLC)

Stock Conc.: 100mM in water, Store at -20℃
Working Conc.: 5mM ATP(Measurement of luciferase activity in extracts from transiently transfected cells.  pH  must be adjusted.)

Store at -20℃