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Molecular Biology Buffer

N,N-Bis[2-Hydroxyethyl]-2-Aminoethanesulfonic Acid
C₆H₁₅NO₅S
M.W.: 213.25
Assay (Titration): 99.78%
A₂₆₀ (1M in water): 0.0600
A₂₈₀ (1M in water): 0.0534

Highly efficient transformation of mammalian cells with a BES-buffered salt solution (50 mM BES, pH 6.95(transformation crucial factor); 280 mM NaCl; 1.5 mM Na₂HPO₄).

Chen, C. & Okayama, H. (1987) Mol. Cell. Biol. 7, 2745-2752
High-efficiency transformation of mammalian cells by plasmid DNA.

Store at Room Temp.

Molecular Biology Buffer

N,N-Bis(Hydroxyethyl)Glycine
C₆H₁₃NO₄
M.W.: 163.17
Assay (Titration): 99.46%
pH(1M in water): 4.8
A₂₆₀ (1M in water): 0.0190
A₂₈₀ (1M in water): 0.0064
White powder

Versatile buffer. Its applications last from enzyme reaction buffers (T4 polynucleotide kinase; Micrococcus nuclease; adenylate cyclase ref. 6) to electrophoresis buffers

Interferes with the BCA and Lowry protein assay.

Working Conc.: 3-100mM

Store at Room Temp.

Molecular Biology Buffer

Bis(2-Hydroxyethyl)-Imino Tris-(Hydroxymethyl)-Methane
C₈H₁₉NO₅
M.W.: 209.24
Assay (Titration): 99.0% Min.

Bis-Tris is an important buffer for protein and nucleic acid systems. Additionally it is used as a substitute for cacodylic acid buffer systems.

Working Conc.: 10-25mM

Store at Room Temp.

Molecular Biology Buffer

C₁₇H₂₆N₈O₅ ·HCl
M.W.: 422.4 . 36.5
Assay: ≥98%

Blasticidin is an antibiotic commonly used in gene selection. Blasticidin is active against both propkaryotic and eukaryotic. Resistance to blasticidin is conferred by the blasticidin resistence gene from bacillus cereus (bsr). Bsr codes for blasticidin deaminase.

Normally, bacteria are sensitive to blasticidin concentrations of 25-100 ug/ml. Mammalian cells demostrate sensitivity at concentrations of 1-10 ug/ml.

Handling: Warning! Toxic. Store in Tightly Sealed Vial. Protect from moisture.

Store at 4℃

Very toxic!

Molecular Biology Buffer, DNA Electrophoresis
Related Product: TBE Buffer

BH₃O₃
M.W.: 61.83
99.5% Min.
Chloride: <0.00042%
Fe: <0.00040%
Sulfate: <0.00014
Loss on Drying: <0.0048%
White crystall powder

For the most commonly used TBE buffer, with a pH of 8.3

Store at Room Temp.