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Molecular Biology Buffer

3-(CyclohexylAmino)-1-PropaneSulfonic Acid
C₉H₁₉NO₃S
M.W.: 221.32
Assay (from N): 99.13%
pKa (25℃): 10.26
Loss on Drying: <0.89%
A₂₅₀ (0.5M in water): 0.0135
A₂₈₀ (0.5M in water): 0.0129

CAPS is an alkaline buffer (pH 10-11, concentration 10-50mM, pH adjustment by NaOH) for protein transfer after SDS polyacrylamide gel electrophoresis on nitrocellulose filters.

Store at Room Temp.

Selection Anitbiotics

Alpha-Carboxybenzylpenicillin Disodium Salt
C₁₇H₁₆N₂O₆S·Na₂
M.W.: 422.36
Assay (Carbenicillin): 92.31%
Benzylpenicillin Na Salt (HPLC): 2.34%
Indineabsorbing Admixtures: 2.59%
Loss on Drying: 3.42%
USP

Carbenicillin is chemicaly similar to ampicillin. It is active against gram negative organism.

When selecting for resistance to ampicillin , transformed cells should be plated at low density (<104 colonies per 90mm plate), and the plates should not be incubated for more than 20hrs at 37℃. The enzyme beta-lactamase is secreted into the medium from ampicillin-resistant transformants and can rapidly inactivate the antibiotic in regions surrounding the colonies. Thus, plating cells at high density or incubating them for long periods of time results in the appearance of ampicillin-sensitive satellite colonies. This problem is ameliorated, but not completely eliminated, by using carbenicillin rather than ampicillin in selective media and increasing the concentration of antibiotic from 60ug/mL to 100ug/mL. The number of ampicillin-resistant colonies does not increase in linear proportion to the number of cells applied to the plate, perhaps because of growth-inhibiting substances released form the cells killed by the antibiotic. (Molecular Cloning, Cold Spring Harbor Laboratory Press, 3rd Edition)

Stock Conc.: 50mg/mL in H₂O/MeOH 1:1, store at -20℃
Working Conc.: 20-60ug/mL

Store at 4℃

Harmful

Selection Antibiotics, Plant Cell Culture

C₁₆H₁₆N₅NaO₇S₂
M.W.: 477.45
Plant cell culture tested.

Non toxic to plant cells. Inhibitor of bacterial cell wall synthesis. Inhibits the crosslinking of peptidoglycan by binding and inactivating transpeptidases. High activity against gram negative bacteria. Often used for elimination of Agrobacterium species after inoculation. Resistant to beta-lactamase activity.

Store at 4℃

DNA/RNA Experiment, For Density Gradient Centrifugation

CsCl
MW:168.36
99.9% Min.
A₂₆₀ (50% w/w H₂O): 0.023
A₂₈₀ (50% w/w H₂O): 0.018

For density gradient centrifugation, No enzymes, Low heavy metals.

Store at Room Temp.

DNA/RNA Experiment, For Density Gradient Centrifugation

CsCl
MW:168.36
99.999% Min.
A₂₆₀ (50% w/w H₂O): 0.009
A₂₈₀ (50% w/w H₂O): 0.008

Highest optical quality for density gradient centrifugation, No enzymes, Low heavy metals.

Store at Room Temp.

Molecular Biology Buffer, A Nondenaturing Zwitterionic Detergent for Membrane Biochemistry

3-[(3-Cholamidopropyl)Dimethylamino]-1-Propanesulfonate
C₃₂H₅₈N₂O₇S
M.W.: 614.88
Assay (from N): 99.94%
TLC: one spot
Loss on Drying: 0.96%
Solubility (10% in water, 25℃): Clear and colorless

CHAPS allows the isolation of many proteins in their native state, without loosing much of its activity. Protein-protein interactions can be interrupted more efficiently as with Triton X-100 or sodium cholate.

CHAPS interferes with the Lowry protein assay.

Working Conc.: 1-13mM

Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-6370
A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis.

Store at 4℃

Molecular Biology Buffer

2-(CycloHexylamino)-Ethane Sulfonic Acid
C₈H₁₇NO₃S
M.W.: 207.29
Assay (Titration): 99.62%
A₂₆₀ (0.1M in water): 0.0078
A₂₈₀ (0.1M in water): 0.0061

CHES interferes with the Lowry assay.

Ellis, K.J. & Morrison, J.F. (1982) Methods Enzymol. 87, 405-426
Buffers of constant ionic strength for studying pH-dependent processes.

Store at Room Temp.

Selection Antibiotics, Cell Culture

C₁₁H₁₂Cl₂N₂O₅
M.W.: 323.13
Assay: 99.2%
Cell culture tested
White or Yellowish-white Crystalline Powder
BP2000
USP24

Bacteriostatic antibiotic with activity against gram-positive and gram-negative bacteria

Stock Conc.: 10 or 34mg/ml in EtOH, Store at -20℃
Working Conc.: 20-170ug/mL

Store at 4℃

Selection Antibiotics

C₁₈H₃₃ClN₂O₅S·HCl·H₂O
M.W.: 479.44

Clindamycin is a lincosamide antibiotic with a primarily bacteriostatic action against gram-positive bacteria.

Store at 4℃

Protein Experiment, Staining Reagent

C₄₇H₅₂N₃NaO₇S₂
M.W.: 858.05

For staining with dye-suspension in TCA solution.
Each tablet contains 50 mg dye and 50 mg salts. Prices refer to product weights.

Store at Room Temp.

Protein Experiment, Staining Reagent

C₄₅H₄₈N₃NaO₇S₂
M.W.: 830.00

For staining with dye-suspension in ethanol-acetic acid solution.
Each tablet contains 50 mg dye and 50 mg salts. Prices refer to product weights

Store at Room Temp.

Streptomyces griseus

C₁₅H₂₃NO₄
M.W.: 281.3
Assay: >98%
Cycloheximide is soluble in Chloroform, Ethanol and Methanol.

Cycloheximide is an antibiotic which acts as an inhibitor of protein synthesis in eukaryotes but not prokaryotes. Cycloheximide is used primarily in the selection wild type CAN1, CYH2 and LYS2 genes.

Lin W, Hsu, Y., Cycloheximide-induced cPLA(2) activation is via the MKP-1 down-regulation and ERK activation., 2000., 12(7):, 457-461..

Handling: Warning! Toxic. Wear gloves/mask when handling this product. Avoid contact by all modes of exposure. Store in Tightly Sealed Vial.

Store at -20℃

Very toxic!

From Fusarium solani

Ciclosporin, Cyclosporin A, Ramihyphin A
C₆₂H₁₁₁N₁₁O₁₂
M.W.: 1202.6
Assay: >99%
Soluble in Methanol, Ethanol, Acetone and Chloroform.

A fungal metabolite with potent immunosuppressive properties.

Handling: Warning! Toxic. Wear gloves/mask when handling product. Avoid contact by all modes of exposure.

Store at 4℃

Very toxic!