Description

Size

Price_USD

For Microbiology Culture

40g for 1L Soln. Components follow:
Tryptone 10g
Yeast Extract 5g
NaCl 10g
Agar 15g

Store at Room Temp.

For Microbiology Culture

25g for 1L Soln. Components follow:
Tryptone 10g
Yeast Extract 5g
NaCl 10g
pH: 7.0±0.05
Bacterial Culture Media
Add 25gm to 1 liter ddH₂O.

Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Store at Room Temp.

For Electrophoresis

Lithium Laurylsulfate
C₁₂H₂₅LiO₄S
M.W.: 272.33
99.5% Min.
Solubility: 25% soluble in water at -5℃

Broglie, R.M. et al. (1980) Proc. Natl. Acad. Sci. USA 77, 87-91
Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecylsulfate/polyacrylamide gel electrophoresis.
Dam, V.T. et al. (1981) Anal. Biochem. 114, 330-335
Separation of poly-(ADP-ribosylated) nuclear proteins by polyacrylamide gel electrophoresis at acidic pH and low temperature.
Kubo, K. & Takagi, T. (1986) Anal. Biochem. 156, 11-16
Binding of Lithium dodecylsulfate to polyacrylamide gel at 4℃ perturbs electrophoresis of proteins.

Store at Room Temp.

Harmful, Irritant

DNA/RNA Experiment, For DNA/RNA Isolation

98% Min.
Activity (25℃): >23,500U/mg
Loss on Drying: 4.1%
pH: 3.6
Sulfated Ash: 0.2%
Nitrogen: 17.6%
Chloride: 2.9%
Heavy Metals: <10ppm

Unit Definition: The amount of enzyme causing a decrease in absorbance at 450nm of 0.001 per minute at 25℃ and pH 6.2 with Micrococcous Lysodeikticus as substrate.

Hydrolyses the beta-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganism. The enzyme is present in many organism. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. Another application is the lysis of bacteria for the preparation of bacterial RNA.

Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, New York.
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. pp. A1.8+4.51. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Store at -20℃. Protect from moisture.