Description

Size

Price_USD

Molecular Biology Buffer

2-(N-Morpholino)EthaneSulfonic acid
C₆H₁₃NO₄S
M.W.: 195.2
99% Min.
Enzyme free
Loss on Drying: <1.0%
Melting Point: >300℃
pKa (25℃): 6.10±0.2
pH (0.5M in water, 20℃): 2.5-4.0

MES is component of e.g. the DNase buffer, does not interfere with the Folin protein assay.

Good, N.E. et al. (1966) Biochemistry 5, 467-477
Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310
Hydrogen ion buffers for biological research.

Store at Room Temp.

Molecular Biology Buffer

2-(N-Morpholino)EthaneSulfonic Acid Monohydrate
C₆H₁₃NO₄S·H₂O
M.W.: 213.26

MES is component of e.g. the DNase buffer, does not interfere with the Folin protein assay.

Good, N.E. et al. (1966) Biochemistry 5, 467-477
Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310
Hydrogen ion buffers for biological research.

Store at Room Temp.

For PCR Use Autoclaved

Autoclaved. Suitable for overlaying aqueous reactions like PCR.

Store at Room Temp.

Selection Antibiotics

Assay: 970ug/mg Min. 
Water: 2.5% Max. 
pH: 6.0-7.5
USD27

Inhibitor of DNA synthesis, nuclear division, and cancer cells. Antibacterial to gram positive, gram negative, acid-fast bacilli.

Tomasz, M., Isolation and structure of a covalent cross-link adduct between mitomycin C and DNA. Science 235, 1204, (1987)
Ueda, M., Sequence-specific DNA damage induced by reduced mitomycin C and 7-N-(p-hydroxyphenyl)mitomycin C. Nucleic Acids Res. 12, 6673, (1984)
Y. Hashimoto et al. Acc. Chem. Res. 17, 403, (1984)

Store at 4℃, protect from light

Very Toxic

Molecular Biology Buffer, RNA Eletrophoresis

3-(N-Morpholino)PropaneSulfonic acid
C₇H₁₅NO₄S
M.W.: 209.26
99% Min.
Loss on Drying: <1.0%
pKa (25℃): 7.15±0.2
pH(10.0% in water 20℃): 3.0-5.0

MOPS interferes with the Folin (Lowry) protein assay, but not the Biuret protein assay. It doesn't bind metal ions and decomposes partially, when autoclaved in the presence of glucose.

Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310
Hydrogen ion buffers for biological research.

Store at Room Temp.

Enzymatic Colorimetric Reagent

Thiazolyl Blue Tetrazolium Bromide
3-(4,5-Dimethyl-Thiazolyl)-2,5-Diphenyl-2H-Tetrazolium Bromide
C₁₈H₁₆N₅SBr
M.W.: 414.32
99% Min.
Red-violet formazan with max. at 565nm
Reagent grade yellow crystals
Loss on Drying: 0.011%
Sulfated Ash: 0.02%
Melting Point: 195-196℃

Mosmann, T. (1983) J. Immunol. Methods 65, 55-63
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays.
Gerlier, D. & Thomasset, N. (1986) J. Immunol. Methods 94, 57-63
Use of MTT colorimetric assay to measure cell activation.
Hansen, M.B. et al. (1989) J. Immunol. Methods 119, 203-210
Re-examination and further development of a precise and rapid dye method for measuring cell growth / cell kill.

Store at 4℃

Enzymatic Colorimetric Reagent for GUS Detection

Fluorescent Substrate of Glucuronidase
4-MethylUmbelliferyl-Beta-D-Glucuronide, Trihydrate
C₁₆H₁₆O₉·3H₂O
M.W.: 406.33
98.5% Min.

Fluorescent substrate of GUS encoded by gusA gene.

Mead, J.A.R. et al. (1955) Biochem. J. 61, 569-574
The biosynthesis of the glucuronides of Umbelliferone and 4-Methylumbelliferone and their use in fluorimetric determination of Beta-Glucuronidase.
Langley, S.D. et al. (1983) J. Biol. Chem. 258, 7416-7424
Genetic variant of Beta-Glucuronidase in Drosophila melanogaster.

Store at -20℃

6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-4-hexenoic acid
C₁₇H₂₀O₆
M.W.: 320.3
Assay: >98%
Soluble in Methane and Methylene Chloride.

Mycophenolic Acid is an antibiotic useful in research for the selection of animal cells that express the E. coli gene coding for XGPRT (xanthine guanine phosphoribosyltransferase). Mycophenolic Acid is also used as immunosuppressive agent becasue it acts by inhibiting lymphocyte proliferation and antibody production.

Merck Index., 2001, 13:, 6352..

Store at Room Temp.

Handling: Warning! Toxic. May be Carcinogenic. Wear gloves/mask when handling product. Avoid contact by all modes of exposure.