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Patulin is a mycotoxin which inhibits the uptake of potassium.

4-Hydroxy-4H-furo[3,2-c]pyran-2(6H)-one, Clavacin
C₇H₆O₄
M.W.: 154.12
Assay: >98%
Patulin is soluble in Dichloromethane.

Merck Index., 12:, 7185., 2. Pfeiffer, E., M. Metzler.. 
Aneuploidpogenic and Clastogenic Potential of the Mycotoxins Citrinin and Patulin., Carcinogenesis., 1998., 19(7):, 1313-1318., 3. Krivobok, S., F. Seigle-Murandi, r. Steiman, J.L. Benoit-Guyod, M.H. Bartoli.. 
Antitumoral Activitiy of Patulin and Patulin-Cysteine Adducts., Pharmazie., 1994., 49(4):, 277-279., 4. Miura, S., K. Hasumi, A. Endo.. Inhihibtion of Protein Prenylation by Patulin., FEBS Letters., 1993., 318(1):, 88-90.

Store at 4℃

Handling: Store in Tightly Sealed Vial. 

Selection Antibiotic

C₁₆H₁₇KN₂O₄S
M.W.: 372.48
Assay(By HPLC): 99.2%
1.6 mega units per gram. 1 BOU ca. 630g. 
Used in cell culture

Penicillin G was isolated from Penicillium notatum. The bactericidal effect of this β-lactam antibiotic is based on the irreversible inhibition of transpeptidase, interfering with the biosynthesis of the cell wall in bacteria.

Working conc.: 50-100ug/mL

Store at 4℃

For PCR Use/High Fidelitiy

5Units/ul.

Purified from the hyperthermophilic archaebacterium Pyrococcus furiosus. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5'=>3' direction in the presence of Mg2+ at 70-80℃. Pfu DNA Polymerase exhibits 3'=>5' exonuclease (proofreading) activity, but has no detectable 5'=>3' exonuclease activity.

Store at -20℃

Molecular Biology Buffer

Piperazine-N, N'-bis[2-ethanesulfonic acids], Free acid. White Powder.
C₈H₁₈N₂O₆S₂
M.W.: 302.37
Assay( Titration): 98.5% min.
Loss on Drying: <0.5%
pKa(20℃): 6.8±0.2
Heavy Metals:<10 ppm
Solubility(0.1M in H₂O): Clear and Complete.

Store at Room Temp.

Protein Experiment Protease Inhibitor

a -Toluenesulfonyl fluoride
C₇H₇FO₂S
M.W.: 174.19
Assay: 99% min.
Melting Point: 91℃
Homogenous by GC and TLC

PMSF is an irreversible inhibitor of serin-proteases like trypsin, chymotrypsin and cysteine proteases. It is frequently used for the preparation of proteins from cell lysates.

Stock conc.: 10 mg/ml in 10% isopropanol, , store at -20°C 
Working conc.: 0.1-1mM 

Fahrney, D.E. & Gold, A.M. (1963) J.Am.Chem. Soc. 85, 996-1000 Sulfonyl fluorides as inhibitors of esterases. I. Rates of reaction with acetylcholinesterase, α-Chymotrypsin and trypsin.
Gold, A.M. & Fahrney, D.E. (1964) Biochemistry 3, 783-791 Sulfonyl fluorides as inhibitors of esterases. II Formation and reactions of phenylmethanesulfonyl α-Chymotrypsin.

Store at Room Temp.

Caution: PMSF is extremely toxic to mucous membranes of the lung, eyes and skin. Any contact by inhalation, swallowing or contact with skin must be avoided. After contact with skin wash immediately with plenty of water. We recommend to use AEBSF instead of PMSF, which is as effective but substantially less toxic. 

Corrosive, Toxic

DNA/RNA Experiment for DNA/RNA Isolation

40milliAnson units/mg
free from nuclease
freeze dryed

Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases.

Stock conc.:10-20mg/mL
Working conc.: 10-100ug/mL

Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York
Sambrook, J., Fritsch, E.F. & Maniatis, T.Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Store at -20℃ 

Irritant, Sensitizing

Selection Antibiotics, Cell Culture

[3’(α-Amino-ρ-methoxyhydrocinnamamido)-3’-deoxy-N,N-dimethyladenosine.2HCl]
C₂₂H₂₉N₇O₅·2HCl
M.W.: 544.43

Antibiotic used for selecting mammalian cell lines ,which have been transformed by vectors that express puromycin-N-acetyl-transferase. 

Puromycin is a aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin.

Stock conc.: 5-50 mg/ml in dH₂O, store at -20℃
Working conc.: 1-30 µg/ml(mammalian cell)

Sambrook, J., Fritsch, E.F. & Maniatis, T.Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 

Store at 4℃

Warning! Toxic. May be Carcinogenic. Wear gloves and mask when handling product. Avoid contact by all modes of exposure.

Toxic