Description

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For Electrophoresis

Dodecyl sulfate sodium salt
C₁₂H₂₅NaO₄S
Measurements of molecular weights by gel electrophoresis.
M.W.: 288.38
Assay: 97.7% min.
Free alcohols:< 0.4%
Homologs:< 1% 
NaCl:< 1% 
Na₂SO₄: <2.5%
pH(10% in water): 5.0

SDS is an anionic detergent which binds nearly to all water-soluble proteins. This binding leads to a conformational change and allows to separate proteins by size. SDS should be used at a concentration of 0.1 % in the gel and in the electrophoresis buffer.

Laemmli, U.K. (1970) Nature 227, 680-685 Cleavage of structural proteins during the assembly of the head of Bacteriophage T4.
Harewood, K. & Wolff III, J.S. (1973) Anal. Biochem. 55, 573-581 A rapid electrophoretic procedure for the detection of SDS-released Oncorna-viral RNA using polyacrylamide-agarose gels.
Nielsen, T.B. & Reynolds, J.A. (1978) Methods Enzymol. 48, 3-10

Store at Room Temp. 

Harmful. Handle the powder in a chemical fume hood. Do not breathe dust. 

Harmful, Irritant

NaCl
M.W.: 58.44
Assay: 99.8% min.
FInsoluble matter: < 0.005%
Loss on Drying: < 0.2%
Bromide (Br): < 0.02%
Iodide (I): < 0.002%
Sulphate (SO₄): < 0.002%
Nitrate (NO₃): < 0.002%
Nitride (N): < 0.001%
Magnesium (Mg): < 0.002%
Potassium (K): < 0.02%
Calcium (Ca): < 0.007%
Iron (Fe): < 3ppm
Arsenic (As): < 0.5ppm
Heavy Metals (as Pb): < 5ppm
pH (20℃, 1M in water): 5.0-8.0(lit.)
Melting Point: 801℃(lit.)
Solubility (20℃, 1M in water):  clear, colorless

Iglewski, B.H., Sadoff, J.C., In gradient elution techniques. Meth. Enzymol. 60, 780, (1979), Fieser 6,285
Hathaway, G.M. Br. Pharm. 60, 495, (1979), Aldrich MSDS 1, 1608:C / Corp MSDS 1 (2), 3135:D / RegBook 1 (3), 3319:F / Sax 6, 2419
Hathaway, G.M. Br. Pharm. 60, 495, (1979), For the preparation of isotonic solutions. Br. Pharm., 742, (1963)
Leckband, D.E. , Role of calcium in the adhesion and fusion of bilayers. Biochemistry 32, 1127, (1993)
Higson, S.P.J., Vadgama, P. Electroanalysis 6, 431, (1994)

Store at Room Temp.

Antibiotic AM-2282, Streptomyces staurosporeus

C₂₈H₂₆N₄O₃
M.W.: 466.5
Assay: >98% 
Staurosporine is soluble in Ethyl Acetate and DMSO. 

Staurosporine is a cell permeable general inhibitor of protein kinases.

Store at 4℃

Handling: Warning! Toxic. May be Carcinogenic. Wear gloves and mask when handling product. Avoid contact by all modes of exposure. Protect from Light.

Very toxic

Selection Antibiotics, Cell Culture

2(C₂₁H₃₉N₇O₁₂)·3H₂SO₄
M.W.: 1457.38

Streptomycin was isolated from Streptomyces griseus. It acts bacteriostatic at low concentrations and bactericidal at higher concentrations against mycobacteria and gram negative bacteria. It is less active against gram positve bacteria. Like other antibiotics, streptomyicin does not act on non-proliferating microorganism. Streptomycin leads to reading error during translation due to the interaction with 23S RNA of the 30S ribosomes.

Stock conc.:10-50mg/mL, store at -20℃. 
Working conc.: 10-100ug/mL

Store at Room Temp. 

Toxic