Description

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For Electrophoresis

Dissolved in ddH₂O. 0.45um filter filtrated.
Ready to use. Easy to use.

Store at Room Temp.

Molecular Biology Buffer

3-[ Tris(hydroxymethyl)methylamino]-1-propane sulfonic acid. C₇H₁₇NO₆S
M.W.: 243.28. 
Assay(Titration): 99.0%min.
pH( 0.1M in H₂O, 25℃): 4.5~6.0
Solubility(5% Soln. in H₂O): Clear and Complete
pKa(25℃): 8.35±0.2

Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research. 

Store at Room Temp.

For PCR Use

5U/uL.
incl. 10X reaction buffer and MgCl₂ solution.
Taq DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from the eubacterium Thermus aquaticus strain YT-1 (1). This unmodified enzyme replicates DNA at 72℃. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5' → 3' direction in the presence of magnesium ions. Besides it possesses a 5' → 3' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA polymerase adds extra 3'-dA nucleotide(s) overhangs to their reaction products.

Kaledin, A.S. et al. (1980) Biokhimiya 45, 644 (Rus)

Store at -20℃

For Electrophoresis

Dissolved in ddH₂O. 0.45um filter filtrated.
Ready to use. Easy to use.

Store at Room Temp.

For Electrophoresis Gel Praperation

Tetramethylethylenediamine , TMEDA
C₆H₁₆N₂
M.W.: 116.21
Assay: 99.9%
pH: 8.0

TEMED was introduced as an enhancer of the polymersitation (cross-linking) of acrylamide and bisacrylamide in gel electrophoresis. It catalysis the formation of free radicals of the initiator of the polymerisation, ammonium persulfate. If gels are degased to remove oxygen, add the TEMED after degasing.

Working conc.: 50uL/100mL

Needles, H.L. (1970) Anal. Biochem. 35, 533-537 Effect of solution components on large-pore polyacrylamide gel formation.
Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.
Gomes, A.V. & Barnes, J.A. (1998) Anal. Biochem. 260, 106-108 Gel electrophoresis of mini gels.

Store at 4℃

Harmful! Handle under a chemical fume hood. 

Harmful, Highly flammable, Corrosive

Molecular Biology Buffer

2-[Tris-(hydroxymethyl)methylamino]-1-ethane sulfonic acid
C₆H₁₅NO₆S
M.W.: 229.25
Assay: 99.0%min.

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.

Store at Room Temp.

Selection Antibiotics, Cell Culture

C₂₂H₂₄N₂O₈·HCl
M.W.: 480.90 
Potency: >900mcg/mg
Moisture: <2.0%
pH(1% in H₂O): 1.8-2.8
4-Enianhydrotetracycline: <2.0%

Tetracycline is a bacteriostatic antibiotic with activity against gram-positive and gram-negative bacteria. Within the cell tetracycline binds reversible to the 30S subunit of the ribosome, preventing the binding of aminoacyl transfer RNA and inhibiting protein synthesis and hence cell growth. Used as a selective marker for the transformation of plasmids encoding for tetracycline resistance (Tetr) such as pBR322, pBR325 and pMB9. 

Stock conc.:12 mg/ml in H₂O
Working conc.: 12 µg/ml

Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M. et al. (1995) Science 268, 1766-1769
Transcriptional activation by Tetracyclines in mammalian cells.

Store at RT. Protected from light!

Molecular Biology Buffer

N-[ Tris-(Hydroxymethyl)-Methyl Glycine
C₆H₁₃NO₅
M.W.: 179.09
Assay(Titration): 99.0%min.
Moisture: 0.62%
pKa(25℃): 7.96
pH(1% in H₂O):5.39
UV(0.1M in H₂O): A260nm/0.0068; B280nm/0.0042. 
White Crystal Powder

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.

Store at Room Temp.

Molecular Biology Buffer, DNA Electrophoresis

Tris-(hydroxymethyl)- aminomethane, 
C₄H₁₁NO₃
M.W.: 121.14
Assay: 99.3% min.
Heavy metals: <10ppm
pH(5% in H₂O): 10.0-11.5
Insoluble Matter: < 0.05%
White Crystalline Powder.

Tris is the most commonly used buffer in biological research. One of the most important applications is the use as an electrophoresis buffer for polyacrylamide and agarose gel electrophoresis, respectively. Tris should not be used at pH values under pH 7.2 or above pH 9.0. The pH value of a Tris buffer strongly depends on the temperature. Therefore, Tris buffers should be prepared at the temperature where it is used.

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.

Store at Room Temp.

Irritant

Molecular Biology Buffer

Tris-(Hydroxymethyl)-aminomethane hydrochloride
C₄H₁₂NO₃Cl
M.W.: 157.60
Assay(Titration): 99%min
Heavy metals(as Pb): <5ppm
pH(0.5M in Water): 3.5-5.0
UV(0.5M in water): A260nm/ < 0.015, A280nm/ < 0.010

Store at Room Temp.

For Microbiology Culture

Total nitrogen TN: 12.9%
pH(2% solution): 6.9
Loss on drying: 4.8%
a-Amino nitrogen AN: 4.1%
Residue on ignition: 11.8% 
Cholride(NaCl): 0.3%
Total aerobic microbial count: 172/g

Tryptone is a component of many bacterial growth rmedia (e. g. LB-Medium, TB-Medium, YT-Medium etc.). It is hygroscopic - Store in a dry place.

Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York. 

Store at Room Temp. Keep Dry!